Central Region
| State | School | Team | City |
|---|---|---|---|
| IA | University of Iowa | IHUC (Iowa Hawkeye Ultimate Club) | Iowa City |
| IA | Iowa State U. | ISUC (Iowa State Ultimate Club) | Ames |
| IA | Luther College | LUFDA | Decorah |
| MN | Carleton C. | CUT | Northfield |
| MN | Winona State University | Experience | Winona |
| MN | St. John’s University | Friar Huck | Collegeville |
| MN | University of Minnesota Morris | Hammertime | Morris |
| MN | St. Cloud State | Luckydog Ultimate | St. Cloud |
| MN | University of Minnesota Duluth | Northern Lights | Duluth |
| MN | University of St. Thomas | Purple Reign | St. Paul |
| MN | Gustavus Adolphus C. | The High | St. Peter |
| MN | University of Minnesota | Ultimate | Twin Cities |
| NE | University of Nebraska | Cornfed | Lincoln |
| NE | University of Nebraska Oamah | UNO | Omaha |
| WI | University of Wisconsin – Milwaukee | Blackcat ultimate | Milwaukee |
| WI | Lawrence U. | Chaos | Appleton |
| WI | Milwaukee School of Engineering | Ducks | Milwaukee |
| WI | University of Wisconsin – Madison | Hodags | Madison |
| WI | University of Wisconsin – Stevens Point | Homegrown | Stevens Point |
| WI | Marquette U. | MU Ultimate | Milwaukee |
| WI | University of Wisconsin – Whitewater | SubPar | Whitewater |
| WI | University of Wisconsin-Platteville | UWP Ultimate | Platteville |